Biodiversity Data Journal Biodiversity Data Journal Biodiversity Data Journal BDJ 1314-2836 1314-2828 Pensoft Publishers Biodiversity Data Journal 10.3897/BDJ.3.e5109 3551 Taxonomic paper Braconidae Genetics Systematics Cenozoic Americas DNA Barcoding of the parasitoid wasp subfamily Doryctinae (Hymenoptera: Braconidae) from Chamela, Mexico Gutiérrez-Arellano Daniela ‡ Gutiérrez-Arellano Claudia Renata § Zaldívar-Riverón Alejandro azaldivar@ib.unam.mx ‡ Colección Nacional de Insectos, Instituto de Biología, Universidad Nacional Autónoma de México, 3er. circuito exterior s/n, Cd. Universitaria, Copilco Coyoacán, A. P. 70-233, C. P. 04510, D. F., Mexico Museo de Zoología “Alfonso L. Herrera”, Facultad de Ciencias, Universidad Nacional Autónoma de México, Apartado Postal 70-399, C. P. 04510, D. F., Mexico

Corresponding author: Alejandro Zaldívar-Riverón (azaldivar@ib.unam.mx).

Academic editor: Jose Fernandez-Triana

 2015 18 05 2015 3 e5109 15 04 2015 13 05 2015 Daniela Gutiérrez-Arellano, Claudia Renata Gutiérrez-Arellano, Alejandro Zaldívar-Riverón This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Background and aims. The Doryctinae is a considerably diverse, poorly studied group of parasitoid wasps and one of the most diverse subfamilies within Braconidae. Taxonomic knowledge of this group remains highly incomplete, specially in the tropics. In Mexico, it has been reported as the subfamily with the highest number of recorded genera. A preliminary Barcoding study carried out in the Chamela region, located near the Mexican pacific coast in Jalisco, identified 185 barcoding species of Dorytinae assigned to 19 identified doryctine genera. This work updates the later study, representing a three years effort to assess the species richness of this subfamily for the Chamela region.

Materials and methods. Ten collecting field trips of 5 to 10 days each were carried out from June 2009 to May 2011. A 2% divergence criterion using the BIN system implemented in BOLD was followed in order to establish species boundaries among the specimens that were collected.

Results and conclusions. A total of 961 specimens were collected, from which 883 COI sequences were obtained. The sequences generated corresponded to 289 barcoding species and 30 identified genera. The most speciose genera were Heterospilus Haliday (170 spp.), Ecphylus Förster (19 spp.), Allorhogas Gahan (15 spp.) and Callihormius Ashmead (14 spp.). Addition of previously collected material increased the diversity of the subfamily in the region to 34 genera and 290 species. Paraphyly of Heterospilus with respect to Neoheterospilus and Heterospathius was again recovered. Twenty new species and two new genera (Sabinita Belokobylskij, Zaldívar-Riverón et Martínez, Ficobolus Martínez, Belokobylskij et Zaldívar-Riverón) have been described so far from the material collected in this work.

 Ichneumonoidea species richness Neotropics faunistic study COI Introduction

Biodiversity inventories represent an integral component for the adequate management of natural resources of any country. In the case of faunistic studies, however, these rarely include arthropod taxa due to their considerably high diversity and scarce taxonomic knowledge. In this context, DNA Barcoding (Hebert et al. 2003a) represents a fast, valuable approach to built species inventories of highly diverse, poorly known invertebrate groups.

With over 1,300 described and almost 3,000 estimated species, Doryctinae is one the four most diverse subfamilies of braconid parasitoid wasps together with the Braconinae, Microgastrinae and Opiinae (Jones et al. 2009, Yu et al. 2012). Doryctine wasps are mainly known to be idiobiont ectoparasitoids of xylophagous and bark boring coleopteran larvae, though other host groups (e.g. Lepidoptera, Diptera, Hymenoptera) and biologies (e.g. phytophagy, endoparasitoidism) have also been reported (Belokobylskij 1992, Belokobylskij et al. 2004). Several doryctine species are known to be parasitoids of insect pests (LaSalle and Gauld 1993), therefore they play an important role for maintaining the balance of terrestrial ecosystems (Hawkins and Hochberg 1994).

The taxonomic knowledge of the Doryctinae is still far from complete, especially in the tropics and subtropics, where most of its species richness is known to occur (Belokobylskij 1992). In the Mexican territory, this was recently reported as the subfamily with the highest number of recorded genera (63 genera; Coronado-Blanco and Zaldívar-Riverón 2014). However, this number contrasts with its scarce number of described species that have been reported for the country. Recently, Zaldívar-Riverón et al. (2010) showed the preliminary results of a DNA Barcoding survey of the Doryctinae from Chamela Biological Station (CBS) in Mexico, which located near the Pacific coast in the state of Jalisco and is mainly composed of tropical dry forest (Noguera et al. 2002). Zaldívar-Riverón et al. (2010)​ not only revealed and extraordinary, mostly undescribed species richness for this group (185 Barcoding and 20 recognized genera), but also showed some previously undetected taxonimic problems.

Here we show the final list of the DNA Barcoding species inventory of the Doryctinae from the CBS, which was carried out during three years of collecting effort. We updated the list of genera that occur in the region and show their number of DNA Barcoding species based on the barcoding index criterion (BIN; Ratnasingham and Hebert 2013). This faunistic study highlights the extraordinary, neglected species richness of this parasitoid group in the Mexican territory, and is also serving as a basis for the subsequent description of several new taxa.

 Materials and methods Study area

Specimens belonging to the subfamily Doryctinae were collected in the Chamela biological station (Fig. 1), owned by the Instituto de Biología, Universidad Nacional Autónoma de México (UNAM). This station is located within the Chamela–Cuixmala Biosphere Reserve (CCBR), near the Pacific coast in the state of Jalisco(19°29' N, 105° 02' W). The prevailing climate in the area is tropical sub-humid (mean annual temperature 24.6 °C, annual precipitation 788 mm; García 1988). There is a strong seasonality in the region, with alternating rainy (Jul- Oct) and dry (Nov-Jun) seasons. Altitude ranges from 0 to 500 meters above sea level. The dominant type of vegetation is tropical dry forest, which is characteristic of the Mexican Pacífic coast, though patches of tropical evergreen forest, coastal dune, xeric shrubland and mangrove are also present (Rzedowski 1978, Noguera et al. 2002). The relevance of this area lies in the variety of habitats it supports, as well as in its high endemic component of plants, vertebrates and insects (Noguera et al. 2002).

 Specimen sampling

A total of 10 collecting trips of 5 to 10 days each were carried out from June 2009 to May 2011. Collects were performed both during the rainy and dry seasons in order to have a complete representation of the examined group through the year. Specimens were collected in 24 sites within the CBS boundaries (Table 1), using for this: 1) malaise traps, 2) light traps, 3) yellow pan traps, as well as 5) sweep nets for at least 4 h per day (Marshall 1994). The collected materials were preserved in 96% ethanol and kept at -20 °C. All specimens were sorted out at subfamily level following the taxonomic key of New World genera of Braconidae (Wharton et al. 1997). Doryctine specimens were then identified to genus level using the relevant literature (Marsh 1997, Marsh 2002) and subsequent descriptions of genera (e.g. Zaldívar-Riverón et al. 2014).

 DNA sequencing

DNA samples were obtained from a single hind leg and sent for DNA extraction and amplification to the Canadian Center for DNA Barcoding at University of Guelph, Ontario (see detailed laboratory protocols in Smith et al. 2008). A 615–658 bp fragment corresponding to the standard animal DNA barcoding locus (cytochrome c oxidase subunit I mtDNA gene, COI) was amplified for the collected samples using both LepF1/LepRI (Hebert et al. 2003b) and LCO1490/HCO2198 (Folmer et al. 1994) primers. Sequences were edited with Sequencher 4.0.5 (Gene Codes Corp.) and aligned manually based on their translated amino acids. All of the COI sequences generated are deposited in GenBank (accession numbers GU715182-288, HM420734-5, HM434309-544, HM882254, HQ200960-201008, HQ201239-54). All sequences and their specimen information are available in the project file “Parasitoid Wasps (Braconidae: Doryctinae) of Chamela–Cuixmala Biosphere Reserve” (ASDORproject) in the projects section of the Barcode of Life Data Systems (www.barcodinglife.com)

 Species boundaries

Species boundaries were established following a 2% divergence criterion (Hebert et al. 2003a). This criterion has been shown to represent a fast, generally reliable tool for exploring species richness in different animal taxa (Ratnasingham and Hebert 2013). This established genetic distance is based on the assumption that COI divergences usually do not exceed a 2% divergence within a recognized species, whereas different species generally show a higher divergence (Hebert et al. 2003a). Sequences divergence of the sequenced specimens were obtained using the K2P distance model (Kimura 1980). A neighbourjoining (NJ) tree and an accumulation curve were reconstructed as implemented in the BOLD system (Barcode of Life Data System, www.boldsystems.org). BINs were obtained for each specimen from the BOLD system, and this was employed to establish the number of barcoding species.

 Data resources

ASDOR Project link

Search as: ASDOR

http://www.boldsystems.org/index.php/Public_BINSearch?searchtype=records

GenBank link

Search as: Nucleotide + accession number

http://www.ncbi.nlm.nih.gov/genbank/

Checklists Checklist of described doryctine species from the Chamela Biological Station Allorhogas coccolobaehttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Martínez and Zaldívar-Riverón 2013 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR472-10; recordedBy: Zaldívar, Zaragoza, Ibarra; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-20-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR517-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.5; decimalLongitude: -105.039; Event: eventDate: 02-21-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR518-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.5; decimalLongitude: -105.039; Event: eventDate: 02-21-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR519-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.5; decimalLongitude: -105.039; Event: eventDate: 02-21-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR764-10; recordedBy: Zaldívar, Salinas, Ramos; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.035; Event: eventDate: 03-28-10

Type status: Holotype. Occurrence: catalogNumber: CNIN777; recordedBy: Zaldívar, Zaragoza, Ibarra; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013)

 Allorhogas crassifemurhttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Martínez and Zaldívar-Riverón 2014 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR042-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: crassifemur; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Holotype. Occurrence: catalogNumber: ASDOR043-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: crassifemur; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-23-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013)

 Allorhogas jaliscoensishttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Martínez and Zaldívar-Riverón 2013 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR081-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 09-24-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR350-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 09-03-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR456-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.038; Event: eventDate: 11-20-09

Type status: Holotype. Occurrence: catalogNumber: ASDOR457-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.038; Event: eventDate: 11-20-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013)

 Allorhogas marshihttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Martínez and Zaldívar-Riverón 2013 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR758-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR759-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR760-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR762-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.496; decimalLongitude: -105.039; Event: eventDate: 03-28-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR763-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.496; decimalLongitude: -105.039; Event: eventDate: 03-28-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR776-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10

Type status: Holotype. Occurrence: catalogNumber: ASDOR777-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013)

 Allorhogas parvushttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Martínez and Zaldívar-Riverón 2013 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR458-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: parvus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 11-21-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR470-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: parvus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-20-10

Type status: Holotype. Occurrence: catalogNumber: ASDOR778-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: parvus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013)

 Allorhogas scottihttp://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Martínez and Zaldívar-Riverón 2013 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR321-10; recordedBy: Clebsch, Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.429; decimalLongitude: -104.98; Event: eventDate: 09-05-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR488-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-20-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR607-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 02-23-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR745-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.496; decimalLongitude: -105.039; Event: eventDate: 03-28-10

Type status: Holotype. Occurrence: catalogNumber: ASDOR767-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.035; Event: eventDate: 03-28-10

Type status: Paratype. Occurrence: catalogNumber: ASDOR768-10; recordedBy: Zaldívar, Salinas, Ramos; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Allorhogas; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.035; Event: eventDate: 03-28-10

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013)

 Ficobolus jaliscoihttp://onlinelibrary.wiley.com/doi/10.1111/syen.12078/abstract;jsessionid=0441E95BA6DBCB93BF1257DF4E1A3B0D.f03t03?deniedAccessCustomisedMessage=&userIsAuthenticated=false Zaldivar-Riverón and Belokobylskij 2014 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR446-10; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Ficobolus; specificEpithet: jaliscoi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-25-09

Type status: Holotype. Occurrence: catalogNumber: ASDOR447-10; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Ficobolus; specificEpithet: jaliscoi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-25-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Zaldívar-Riverón et al. 2014)

 Heerz ecmahlahttp://zookeys.pensoft.net/articles.php?id=2399 Martínez, Zaldívar-Riverón, Ceccarelli and Shaw 2012 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR075-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Heerz; specificEpithet: ecmahla; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Holotype. Occurrence: catalogNumber: ASDOR076-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Heerz; specificEpithet: ecmahla; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012)

 Heerz macrophthalmahttp://zookeys.pensoft.net/articles.php?id=2400 Martínez, Zaldívar-Riverón, Ceccarelli and Shaw 2013 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR551-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Heerz; specificEpithet: macrophthalma; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 02-02-11

Type status: Paratype. Occurrence: catalogNumber: ASDOR555-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Heerz; specificEpithet: macrophthalma; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 02-02-11

Type status: Paratype. Occurrence: catalogNumber: ASDOR761-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Heerz; specificEpithet: macrophthalma; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10

Type status: Holotype. Occurrence: catalogNumber: CNIN795; recordedBy: Zaldívar, Zaragoza, Ibarra; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Heerz; specificEpithet: macrophthalma; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012)

 Iare belokobylskijihttp://ejournal.narotama.ac.id/files/The%20genus%20Iare%20Barbalho%20and%20Penteado-Dias%20(Hymenoptera%20Braconidae%20Doryctinae)%20in%20Mexico,%20with%20the%20description%20of%20two%20new%20species.pdf Marsh 2002 Materials

Type status: Other material. Occurrence: catalogNumber: ASDOR022-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Other material. Occurrence: catalogNumber: ASDOR023-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Other material. Occurrence: catalogNumber: ASDOR025-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Other material. Occurrence: catalogNumber: ASDOR026-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Other material. Occurrence: catalogNumber: ASDOR029-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Other material. Occurrence: catalogNumber: ASDOR031-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Other material. Occurrence: catalogNumber: ASDOR035-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

 Distribution

Mexico and Costa Rica

 Iare cheguevaraihttp://ejournal.narotama.ac.id/files/The%20genus%20Iare%20Barbalho%20and%20Penteado-Dias%20(Hymenoptera%20Braconidae%20Doryctinae)%20in%20Mexico,%20with%20the%20description%20of%20two%20new%20species.pdf Martínez, Zaldívar-Riverón, Ceccarelli and Shaw 2010 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR020-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR021-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR027-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Holotype. Occurrence: catalogNumber: ASDOR028-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR096-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez et al. 2010)

 Iare mexicanushttp://ejournal.narotama.ac.id/files/The%20genus%20Iare%20Barbalho%20and%20Penteado-Dias%20(Hymenoptera%20Braconidae%20Doryctinae)%20in%20Mexico,%20with%20the%20description%20of%20two%20new%20species.pdf Martínez, Zaldívar-Riverón, Ceccarelli and Shaw 2011 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR030-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: mexicanus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Holotype. Occurrence: catalogNumber: ASDOR033-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Iare; specificEpithet: mexicanus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez et al. 2010)

 Lissopsius jaliscoensishttp://zookeys.pensoft.net/articles.php?id=2399 Zaldívar-Riverón, Martínez, Ceccarelli and Shaw 2012 Materials

Type status: Paratype. Occurrence: catalogNumber: CNIN741; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Holotype. Occurrence: catalogNumber: CNIN798; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-27-09

Type status: Paratype. Occurrence: catalogNumber: CNIN798; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Paratype. Occurrence: catalogNumber: CNIN799; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-24-09

Type status: Paratype. Occurrence: catalogNumber: CNIN800; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-24-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012)

 Lissopsius pacificushttp://zookeys.pensoft.net/articles.php?id=2399 Zaldívar-Riverón, Martínez, ceccarelli and Shaw 2013 Materials

Type status: Paratype. Occurrence: catalogNumber: CNIN739; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: pacificus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11

Type status: Holotype. Occurrence: catalogNumber: CNIN740; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: pacificus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-26-09

Type status: Paratype. Occurrence: catalogNumber: CNIN742; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: pacificus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11

Type status: Paratype. Occurrence: catalogNumber: CNIN743; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Lissopsius; specificEpithet: pacificus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012)

 Monarea fridaehttp://dx.doi.org/10.11646/zootaxa.3795.4.2 Belokobylskij, Zaldivar-Riverón, Coronado-Blanco 2014 Materials

Type status: Holotype. Occurrence: recordedBy: Toledo; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Monarea; specificEpithet: fridae; Location: country: Mexico; stateProvince: Morelos; municipality: Tepalcingo; locality: El Limón; decimalLatitude: 12.52; decimalLongitude: -98.94; Event: eventDate: 10-13-12

Type status: Paratype. Occurrence: recordedBy: Toledo, Hinterholzer, Martínez; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Monarea; specificEpithet: fridae; Location: country: Mexico; stateProvince: Puebla; municipality: Jolalpan; locality: Rancho el Salado; decimalLatitude: 18.33; decimalLongitude: -98.98; Event: eventDate: 10-07-10

Type status: Paratype. Occurrence: recordedBy: Toledo; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Monarea; specificEpithet: fridae; Location: country: Mexico; stateProvince: Morelos; municipality: Tlaquiltenango; locality: Santiopa; decimalLatitude: 18.44; decimalLongitude: -98.95; Event: eventDate: 07-06-13

Type status: Paratype. Occurrence: recordedBy: Toledo; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Monarea; specificEpithet: fridae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.49; decimalLongitude: -105.044; Event: eventDate: 06-06-11

 Distribution

Central Mexico and Jalisco

 Notes

n.sp. described from specimens collected in this study (Belokobyskij et al. 2014)

 Neoheterospilus chamelaehttp://www.conabio.gob.mx/institucion/proyectos/resultados/HB033_Neoheterospilus%202010.pdf Marínez and Zaldívar-Riverón 2010 Materials

Type status: Holotype. Occurrence: catalogNumber: ASDOR053-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044

Type status: Paratype. Occurrence: catalogNumber: ASDOR054-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044

Type status: Paratype. Occurrence: catalogNumber: ASDOR095-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038

Type status: Other material. Occurrence: catalogNumber: ASDOR108-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038

Type status: Other material. Occurrence: catalogNumber: ASDOR148-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.497; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR202-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR216-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.045

Type status: Paratype. Occurrence: catalogNumber: ASDOR343-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.429; decimalLongitude: -104.98

Type status: Paratype. Occurrence: catalogNumber: ASDOR392-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044

Type status: Paratype. Occurrence: catalogNumber: ASDOR418-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR473-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042

Type status: Paratype. Occurrence: catalogNumber: ASDOR482-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042

Type status: Paratype. Occurrence: catalogNumber: ASDOR492-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042

Type status: Paratype. Occurrence: catalogNumber: ASDOR549-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.509; decimalLongitude: -105.037

Type status: Paratype. Occurrence: catalogNumber: ASDOR550-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.509; decimalLongitude: -105.037

Type status: Paratype. Occurrence: catalogNumber: ASDOR565-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044

Type status: Paratype. Occurrence: catalogNumber: ASDOR566-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044

Type status: Paratype. Occurrence: catalogNumber: ASDOR567-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044

Type status: Paratype. Occurrence: catalogNumber: ASDOR601-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR625-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR627-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR632-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR633-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR697-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042

Type status: Other material. Occurrence: catalogNumber: ASDOR718-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.035

Type status: Other material. Occurrence: catalogNumber: ASDOR737-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.496; decimalLongitude: -105.039

Type status: Other material. Occurrence: catalogNumber: ASDOR769-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042

Type status: Other material. Occurrence: catalogNumber: ASDOR770-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042

Type status: Other material. Occurrence: catalogNumber: ASDOR772-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.5; decimalLongitude: -105.039

Type status: Other material. Occurrence: catalogNumber: ASDOR773-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038

Type status: Other material. Occurrence: catalogNumber: ASDOR775-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038

Type status: Other material. Occurrence: catalogNumber: ASDOR789-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044

Type status: Other material. Occurrence: catalogNumber: ASDOR816-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044

Type status: Other material. Occurrence: catalogNumber: ASDOR820-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044

Type status: Other material. Occurrence: catalogNumber: ASDOR822-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.49; decimalLongitude: -105.038

Type status: Other material. Occurrence: catalogNumber: ASDOR853-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Neoheterospilus; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2010)

 Notiospathius crypticushttp://www.ib.unam.mx/m/revista/pdfs/05.-_1581_1.pdf Reséndiz-Flores, Nunes and Zaldívar Riverón 2014 Materials

Type status: Holotype. Occurrence: catalogNumber: ASDOR016-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Notiospathius; specificEpithet: crypticus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-25-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR017-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Notiospathius; specificEpithet: crypticus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-26-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Reséndiz-Flores et al. 2014)

 Notiospathius mariachihttp://www.ib.unam.mx/m/revista/pdfs/05.-_1581_1.pdf Reséndiz-Flores, Nunes and Zaldívar Riverón 2014 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR018-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Notiospathius; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-26-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR019-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Notiospathius; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-27-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR355-10; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Notiospathius; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 09-04-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR357-10; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Notiospathius; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 09-03-09

Type status: Holotype. Occurrence: catalogNumber: ASDOR463-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Notiospathius; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.038; Event: eventDate: 11-20-09

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Reséndiz-Flores et al. 2014)

 Ondigus cuixmalensishttp://zookeys.pensoft.net/articles.php?id=2399 Zaldívar-Riverón, Martínez, ceccarelli and Shaw 2014 Materials

Type status: Holotype. Occurrence: catalogNumber: ASDOR464-10; recordedBy: Clebsch, Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Ondigus; specificEpithet: cuixmalensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.419; decimalLongitude: -104.973; Event: eventDate: 09-03-09

Type status: Paratype. Occurrence: catalogNumber: ASDOR514-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Ondigus; specificEpithet: cuixmalensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 02-20-10

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012)

 Sabinita mexicanahttp://onlinelibrary.wiley.com/doi/10.1111/syen.12078/abstract;jsessionid=0441E95BA6DBCB93BF1257DF4E1A3B0D.f03t03?deniedAccessCustomisedMessage=&userIsAuthenticated=false Belokobylskij, Zaldivar-Riverón and Martínez 2014 Materials

Type status: Holotype. Occurrence: catalogNumber: ASDOR082-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Sabinita; specificEpithet: mexicana; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.497; decimalLongitude: -105.038; Event: eventDate: 07-02-12

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Zaldívar-Riverón et al. 2014)

 Spathius chamelaehttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137313/pdf/zookeys-427-059.pdf Belokobylskij and Zaldivar-Riverón 2014 Materials

Type status: Paratype. Occurrence: catalogNumber: ASDOR371-10; recordedBy: Clebsch, Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Spathius; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR372-10; recordedBy: Clebsch, Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Spathius; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR373-10; recordedBy: Clebsch, Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Spathius; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR375-10; recordedBy: Clebsch, Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Spathius; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Paratype. Occurrence: catalogNumber: ASDOR432-10; recordedBy: Clebsch, Zaldívar; sex: male; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Spathius; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

Type status: Holotype. Occurrence: catalogNumber: ASDOR433-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Spathius; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038

 Distribution

Chamela, Jalisco, Mexico

 Notes

n.sp. described from specimens collected in this study (Belokobylskij and Zaldivar-Riveron 2014)

 Whitfieldiellus variegatahttp://doryctinaekey.myspecies.info/file/339 Marsh 1993 Materials

Type status: Other material. Occurrence: catalogNumber: ASDOR001-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: Animalia; phylum: Arthropoda; class: Insecta; order: Hymenoptera; family: Braconidae; genus: Whitfieldiellus; specificEpithet: variegata; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09

 Distribution

Mexico, Guatemala, Panama and Costa Rica

 Analysis Species boundaries

A total of 961 specimens were collected during the ten field trips, of which 883 COI sequences were generated. Fifteen of these sequences had a length lower than 500 bp and thus did not receive a BIN, though they were included in the analysis. The sequences generated belonged to 289 barcoding species and 30 identified genera, though four barcoding species could not be assigned to any genus (Table 2). Heterospilus Haliday was the most speciose genera with 170 barcoding species, followed by Ecphylus Förster (19 spp.), Allorhogas Gahan (15 spp.) and Callihormius Ashmead (14 spp.). Two species belonging to two additional genera (Doryctinus Roman; Monarea fridae, Belokobyskij et al. 2014 were foundfound in the entomological collection at the CBS, increasing the diversity of doryctine genera and species present in the region to 33 and 289, respectively.

The reconstructed NJ tree recovered the megadiverse genus Heterospilus as non-monophyletic with respect to Heterospathius and Neotherospilus (Fig. 2). The accumulation curve estimations of species richness (Fig. 3) indicates that the number of species that occur in the region has not been reached.

Twenty new species and two new genera (Sabinita Belokobylskij, Zaldívar-Riverón et Martínez, Ficobolus Martínez, Belokobylskij et Zaldívar-Riverón) have been described by AZR and collaborators from the doryctine specimens collected for this study (Table 3). An update on Zaldívar-Riverón et al. 2010) generic identification was performed. Specimens assigned to Hansonorum Marsh were transferred to Notiospathius, since the former genus is now considered its junior synonym (De Jesús-Bonilla et al. 2011). Moreover, the specimens assigned in the above study to Barbalhoa Marsh and Platydoryctes Barbalho et Penteado-Dias actually belong to Concurtisella and Callihormius, respectively.

 Discussion

The Mexican dry tropical forest is known for containing a considerably high species richness and endemicity rates for various plant and animal groups (Ceballos et al. 2010). Despite that the current available information does not allow to determine what proportion of the richness registered for insects in Mexico occurs in dry tropical forests, the gathered data for other taxa suggest that it is considerably high (Zaragoza-Caballero et al. 2010). This work represents one of various DNA barcoding species inventories that are being carried out for selected braconid subfamilies (Agathidinae, Braconinae, Rogadinae) and for other insect taxa (e.g. Coleoptera: Cerambycidae, Elateridae) in the CBS, one of which has already been published (Microgastrinae, Fernández-Flores et al. 2013).

Our updated study identified 14 additional genera and increased 53% the number of barcoding species found in the CBS with respect to the results obtained in Zaldívar-Riverón et al. 2010 preliminary study. Though most of the doryctine species that were discriminated remain undescribed, 20 of them and two new genera (Sabinita and Ficobolus) were already described by AZR and collaborators based on the material collected in this work. Most of these new described species belong to small, poorly known genera. The published records for the Chamela region indicate that the species richness of the Doryctinae is considerably higher than the those observed for Microgastrinae (103 spp.; Fernández-Flores et al. 2013), considered to be the second largest subfamily of Braconidae (Jones et al. 2009), and Rogadinae (27 spp.; Aguilar-Velasco 2013).

A vast species richness found for the subfamily Doryctinae was reported for Costa Rica (458 spp.; Marsh 2002, Marsh et al. 2013). This species richness, however, was reported for the whole country, whereas in our study the species richness found for the subfamily (290 spp.) was limited to about 3,000 ha. Particularly, we delimited 170 barcoding species for the genus Heterospilus for the CBS, whereas for Costa Rica, Marsh et al. 2013 described 280 species (Marsh et al. 2013).

Similar to our preliminary study, paraphyly of Heterospilus with respect to Heterospathius and Neoheterospilus was again recovered. A non-monophyletic Heterospilus was also recovered in a recent multi locus phylogenetic study (Wild et al. 2013). Our results therefore support that, though it is not appropriate to reconstruct phylogenetic relationships only based on a single mitochondrial marker, barcoding data represents an accessible, comprehensive system for species identification (Hebert and Gregory 2005).

 References Aguilar-Velasco R. G. 2013 Diversidad de especies y asociación de parasitoidismo en las avispas de la subfamilia Rogadinae (Hymenoptera: Braconidae) de la Estación de Biología de Chamela, Jalisco. BSc Thesis. Facultad de Ciencias Universidad Nacional Autónoma de México D.F 67 Belokobylskij SA 1992 On the classification and phylogeny of the Braconid wasps subfamilies Doryctinae and Exothecinae (Hymenoptera, Braconidae). Part I. On the classification, 1 Entomologicheskoe Obozrenie 71 900 928 Belokobylskij Sergey Zaldivar-Riveron Alejandro 2014 The genus Spathius Nees (Hymenoptera, Braconidae, Doryctinae) in Mexico: occurrence of a highly diverse Old World taxon in the Neotropics ZooKeys 427 59 73 http://dx.doi.org/10.3897/zookeys.427.8074 10.3897/zookeys.427.8074 Belokobylskij S. A. Zaldívar-Riverón A. Quicke D. L. J. 2004 Phylogeny of the genera of the parasitic wasps subfamily Doryctinae (Hymenoptera: Braconidae) based on morphological evidence Zoological Journal of the Linnean Society 142 3 369 404 10.1111/j.1096-3642.2004.00133.x Belokobyskij S A Zaldívar-Riverón A Coronado-Blanco J M 2014 Phylogenetic affinities of Monarea Szépligeti, 1904 (Hymenoptera: Braconidae, Doryctinae, with description of a new species from Mexico Zootaxa 3795 4 421 http://dx.doi.org/10.11646/zootaxa.3795.4.2 10.11646/zootaxa.3795.4.2 Ceballos G Martínez L García A Espinoza E Bezaury-Creel J Dirzo R 2010 Diversidad, amenazas y áreas prioritarias para la conservación de las Selvas Secas del Pacífico de México Fondo de Cultura Económica and Comisión Nacional para el Conocimiento y Uso de la Biodiversidad México, D.F. 596 970-9000-38-1 Coronado-Blanco J. M. Zaldívar-Riverón A. 2014 Biodiversidad de Braconidae (Hymenoptera: Ichneumonoidea) en México Revista Mexicana de Biodiversidad 85 S372 S378 10.7550/rmb.32000 De Jesús-Bonilla Vladimir Salvador Nunes Juliano Penteado-Días Angélica Csösz Sándor Zaldívar-Riverón Alejandro 2011 A new synonym of the Neotropical parasitoid wasp genus Notiospathius (Braconidae, Doryctinae), with redescription of two species and description of five new species from Brazil ZooKeys 122 71 90 http://dx.doi.org/10.3897/zookeys.122.1243 10.3897/zookeys.122.1243 Fernández-Flores S. Fernández-Triana J. L. Martínez J. J. Zaldívar-Riverón A. 2013 DNA barcoding species inventory of Microgastrinae wasps (Hymenoptera, Braconidae) from a Mexican tropical dry forest Molecular Ecology Resources 13 1146 1150 http://dx.doi.org/10.1111/1755-0998.12102 10.1111/1755-0998.12102 Folmer O Black M Hoeh W Lutz R Vrijenhoek RC 1994 DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3 294 299 García Enriqueta 1988 Modificaciones al sistema de classificación climática de Köppen UNAM México, D.F 217 Hawkins BA Hochberg ME 1994 The implications of population dynamics theory to parasitoids diversity and biological control Hawkins Bradford A. Sheehan William Parasitoid community ecology Oxford University Press Oxford 20 Hebert Paul Gregory T. Ryan 2005 The Promise of DNA Barcoding for Taxonomy Systematic Biology 54 5 852 859 http://dx.doi.org/10.1080/10635150500354886 10.1080/10635150500354886 Hebert Paul D N Ratnasingham Sujeevan deWaard Jeremy R 2003 Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings. Biological sciences / The Royal Society 270 96 9 http://dx.doi.org/10.1098/rsbl.2003.0025 10.1098/rsbl.2003.0025 Hebert P. D. N. Cywinska A. Ball S. L. deWaard J. R. 2003 Biological identifications through DNA barcodes Proceedings of the Royal Society B: Biological Sciences 270 1512 313 321 10.1098/rspb.2002.2218 Jones OR Purvis Andy Baumgart E Quicke DLJ 2009 Using taxonomic revision data to estimate the geographic and taxonomic distribution of undescribed species richness in the Braconidae (Hymenoptera: Ichneumonoidea) Insect Conservation and Diversity 2 3 204 212 http://dx.doi.org/10.1111/j.1752-4598.2009.00057.x 10.1111/j.1752-4598.2009.00057.x Kimura Motoo 1980 A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences Journal of Molecular Evolution 16 2 111 120 http://dx.doi.org/10.1007/bf01731581 10.1007/bf01731581 LaSalle J. Gauld I. D. 1993 Hymenoptera and biodiversity CAB International Wallingford 348 085198830X Marshall Steven A. 1994 Terrestrial Arthropod Biodiversity: Planning a Study and Recommended Sampling Techniques: a Brief Entomological Society of Canada Bulletin 26 1 1 33 10.4039/entm126169fv Marsh PM 2002 The Doryctinae of Costa Rica (excluding the genus Heterospilus). Memoirs of the American Entomological Institute 70 1 319 Marsh Paul Wild Alexander Whitfield James 2013 The Doryctinae (Braconidae) of Costa Rica: genera and species of the tribe Heterospilini ZooKeys 347 1 474 http://dx.doi.org/10.3897/zookeys.347.6002 10.3897/zookeys.347.6002 Marsh P. M. 1997 Doryctinae Wharton R. A. Marsh P. M. Sharkey M. J. Manual of the New World genera of the Family Braconidae (Hymenoptera) International Society of Hymenopterists Washington, D.C 438 Martínez Juan J. Zaldívar-Riverón Alejandro 2010 A new species of Neoheterospilus (Hymenoptera: Braconidae: Doryctinae) from Chamela, Jalisco, Mexico Journal of Hymenoptera Research 19 217 222 Martínez Juan José Zaldívar-Riverón Alejandro 2013 Seven new species of Allorhogas (Hymenoptera: Braconidae: Doryctinae) from Mexico Revista Mexicana de Biodiversidad 84 1 117 139 http://dx.doi.org/10.7550/rmb.31955 10.7550/rmb.31955 Martínez J J Ceccarelli F S Zaldívar-Riverón A 2010 The genus Iare Barbalho and Penteado-Dias (Hymenoptera: Braconidae: Doryctinae) in Mexico, with the description of two new species Zootaxa 2685 30 38 Noguera Felipe A. Vega JH García AN Quesada M 2002 Historia natural de Chamela Instituto de Biología, UNAM México, D.F xv-xxi 9703205208 Ratnasingham Sujeevan Hebert Paul D. N. 2013 A DNA-Based Registry for All Animal Species: The Barcode Index Number (BIN) System PLoS ONE 8 7 e66213 10.1371/journal.pone.0066213 Reséndiz-Flores Andrés Nunes Juliano F. García-París Mario Zaldívar-Riverón Alejandro 2014 Six new species of the parasitoid wasp genus Notiospathius (Hymenoptera: Braconidae: Doryctinae) from Mexico Revista Mexicana de Biodiversidad 85 2 391 401 http://dx.doi.org/10.7550/rmb.41972 10.7550/rmb.41972 Rzedowski Jerzy 1978 Vegetación de México Limusa México, D.F 432 Smith M. A. Rodriguez J. J. Whitfield J. B. Deans A. R. Janzen D. H. Hallwachs W. Hebert P. D. N. 2008 Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections Proceedings of the National Academy of Sciences 105 34 12359 12364 10.1073/pnas.0805319105 Wharton Robert A. Marsh Paul M. Sharkey Michael J. 1997 Manual of the New World genera of the family Braconidae (Hymenoptera) International Society of Hymenopterists Washington, D.C Wild Alexander L. Marsh Paul M. Whitfield James B. 2013 Fast-Evolving Homoplastic Traits Are Best for Species Identification in a Group of Neotropical Wasps PLoS ONE 8 9 e74837 http://dx.doi.org/10.1371/journal.pone.0074837 10.1371/journal.pone.0074837 Yu DSK van Achterberg C Horstmann K 2012 Taxapad 2012, Ichneumonoidea 2011. Database on flash-drive. www.taxapad.com. Ottawa, Ontario, Canada Zaldivar-Riveron Alejandro Martínez Juan Ceccarelli Fadia Shaw Scott 2012 Five new species of the genera Heerz Marsh, Lissopsius Marsh and Ondigus Braet, Barbalho and van Achterberg (Braconidae, Doryctinae) from the Chamela-Cuixmala biosphere reserve in Jalisco, Mexico ZooKeys 164 1 23 http://dx.doi.org/10.3897/zookeys.164.2201 10.3897/zookeys.164.2201 Zaldívar-Riverón Alejandro Martínez Juan J. Belokobylkij Sergey A. Pedraza-Lara Carlos Shaw Scott R. Hanson Paul E. Varela-Hernández Fernando 2014 Systematics and evolution of gall formation in the plant-associated genera of the wasp subfamily Doryctinae (Hymenoptera: Braconidae) Systematic Entomology 39 4 633 659 http://dx.doi.org/10.1111/syen.12078 10.1111/syen.12078 Zaldívar-Riverón Alejandro Martínez Juan José Ceccarelli Fadia Sara De Jesús-Bonilla Vladimir Salvador Rodríguez-Pérez Ana Cecilia Reséndiz-Flores Andrés Smith M. Alex 2010 DNA barcoding a highly diverse group of parasitoid wasps (Braconidae: Doryctinae) from a Mexican nature reserve Mitochondrial DNA 21 18 23 10.3109/19401736.2010.523701 Zaragoza-Caballero S Noguera FA González-Soriano E Ramírez-García E Rodríguez-Palafox A 2010 Insectos Ceballos G Martínez L García A Espinosa E Bezaury-Creel J Dirzo R Diversidad, amenazas y áreas prioritarias para la conservación de las selvas secas del Pacífico de México Fondo de Cultura Económica and Comisión Nacional para el Conocimiento y Uso de la Biodiversidad México, D.F 596 970-9000-38-1

Study area. The Chamela Biological Station (IB-UNAM), located within the Chamela-Cuixmala Biosphere Reserve in the estate of Jalisco, Mexico.

Neighbour-joining tree obtained from BOLD that includes 883 nucleotide sequences belonging to doryctine specimens. The distance model used was the Kimura 2 Parameter, the marker was COI-5P, and the 1st, 2nd and 3rd codon positions were included. The sequences had a lengrh greater than 200bp. See Suppl. material 1.

DNA barcoding species accumulation curve for the Doryctinae from the CBS (Suppl. material 1).

Sampling sites and collecting techniques employed in this study.

SITE LATITUDE LONGITUDE ALTITUDE TRAP TYPE
Near CBS 19.4985 -105.0441 92 Yellow pan trap
Camino Zarco/Chachalaca 19.4956 -105.0393 30 Sweep net
Camino Búho 19.4990 -105.0412 74 Malaise
19.4988 -105.0404 65 Light trap
Camino Chachalaca 19.4993 -105.0383 56 Sweep net, Malise trap, Yellow pan trap
19.4978 -105.0445 120 Sweep net
Camino Calandria 19.5084 -105.0378 120 Yellow pan trap, sweep net, light trap, Malaise trap
Eje central/Camino Calandria 19.5002 -105.0425 49 Yellow pan
19.5039 -105.0335 52 Sweep net
Camino Calandria/Camino Chachalaca 19.5049 -105.0377 52 Sweep net
Fundación-Cuixmala/Poza del Jaguar 19.4292 -104.9796 66 Sweep net
Fundación-Cuixmala/El Sendero 19.4192 -104.9732 61 Sweep net, Malaise trap
Behind CBS dinning room 19.4978 -105.0445 120 Sweep net
Camino Ardilla 19.5042 -105.0379 39 Sweep net
Eje central 19.5085 -105.0366 32 Sweep net
Calandria(Arroyo Calandria) 19.5036 -105.0364 62 Sweep net
19.5002 -105.0353 109 Sweep net
Camino Búho/ Chachalaca 19.4991 -105.0421 68 "O" Trap
19.4978 -105.0444 106 Sweep net
Camino Antiguo 19.5047 -105.0367 59 Sweep net

Doryctine genera and number of barcoding species identified in this study (Total 289).

* Species identified only based on morphological evidence.

Genera Barcoding species
Allorhogas Gahan 15
Caenophanes Förster 1
Callihormius Ashmead 14
Coiba Marsh 7
Concurtisella Roman 1
Curtisella Spinola 4
Doryctinus* Roman 1
Ecphylus Förster 19
Ficobolus Martínez, Belokobylskij et Zaldívar-Riverón 1
Glyptocolastes Ashmead 8
Heerz Marsh 2
Heterospathius Barbalho et Penteado-Dias 1
Heterospilus Haliday 170
Iare Barbalho et Penteado-Dias 6
Janzenia Marsh 1
Leluthia Cameron 1
Lissopsius Marsh 2
Masonius Marsh 2
Monarea* Szépligeti 1
Neoheterospilus Belokobylskij 3
Nervellius Roman 1
Notiospathius Matthews et Marsh 5
Odontobracon Cameron 2
Ondigus Braet, Barbalhoa et Van Achterberg 2
Panama Marsh 1
Psenobolus Reinhard 2
Ptesimogaster Marsh 1
Rhaconotus Ruthe 3
Sabinita Belokobylskij, Zaldívar-Riverón et Martínez 1
Spathius Nees 1
Stenocorse Marsh 3
Vanderentiellus Marsh 2
Whitfieldiellus Marsh 1
Unidentifed 4

List of the new species described from specimens collected in this study

SPECIES REFERENCE
Allorhogas coccolobae Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas crassifemur Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas jaliscoensis Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas marshi Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas parvus Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas scotti Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Ficobolus jaliscoi Zaldívar-Riverón and Belokobylskij Martínez and Zaldívar-Riverón 2013, Zaldívar-Riverón et al. 2014
Heerz ecmahla Martínez, Zaldívar-Riverón, Ceccarelli and Shaw Zaldivar-Riveron et al. 2012
Heerz macrophthalma Martínez, Zaldívar-Riverón, Ceccarelli and Shaw Zaldivar-Riveron et al. 2012
Iare cheguevarai Martínez, Zaldívar-Riverón and Ceccarelli Martínez et al. 2010
Iare mexicanus Martínez, Zaldívar-Riverón and Ceccarelli Martínez et al. 2010
Lissopsius jaliscoensis Zaldívar-Riverón, Martínez, Ceccarelli et Shaw Zaldivar-Riveron et al. 2012
Lissopsius pacificus Zaldívar-Riverón, Martínez, Ceccarelli et Shaw Zaldivar-Riveron et al. 2012
Monarea fridae Belokobylskij, Zaldivar-Riveron et Coronado-Blanco Belokobyskij et al. 2014
Neoheterospilus chamelae Martínez et Zaldívar-Riverón Martínez and Zaldívar-Riverón 2010
Notiospathius crypticus Reséndiz-Flores, Nunes and Zaldívar-Riverón Reséndiz-Flores et al. 2014
Notiospathius mariachi Reséndiz-Flores, Nunes and Zaldívar-Riverón Reséndiz-Flores et al. 2014
Ondigus cuixmalensis Zaldívar-Riverón, Martínez, Ceccarelli and Shaw Zaldivar-Riveron et al. 2012
Sabinita mexicana Belokobylskij, Zaldívar-Riverón and Martínez Zaldívar-Riverón et al. 2014
Spathius chamelae Belokobylskij and Zaldívar-Riverón Belokobylskij and Zaldivar-Riveron 2014

Accumulation Curve Data

Data type: Table of records

Brief description: Table containing the Process ID of specimens sampled and Barcode Index Number (BIN) used for the species accumulation curve.

File: oo_40543.xls

A. Zaldívar-Riverón, C.R. Gutiérrez-Arellano, D. Gutiérrez-Arellano