Biodiversity Data JournalBiodiversity Data JournalBiodiversity Data JournalBDJ1314-28361314-2828Pensoft PublishersBiodiversity Data Journal10.3897/BDJ.3.e44513883Taxonomic paperAraneaeTetragnathidaeBiodiversity: Species, Ecosystems & ConservationTaxonomyAsiaDescription of male Tyloridasataraensis Kulkarni, 2014 (Araneae, Tetragnathidae) with notes on habits and conservation statusKulkarniSiddharth Shrikantsskspider@gmail.com†‡LewisTodd R§Biome Conservation Foundation, Pune, IndiaZoology Department, Yashavantrao Chavan Institute of Science, Satara, Satara, IndiaWestfield, 4 Worgret Road, Wareham, Dorset, BH20 4PJ., Dorset, United Kingdom
2015160220153e44510501201513022015Siddharth Shrikant Kulkarni, Todd R LewisThis is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The male sex of Tyloridasataraensis Kulkarni, 2014 is described based on specimens from the type locality. The distinguishing characters from its closest species Tyloridaventralis (Thorell, 1877) are detailed. An interesting behaviour of going underwater by T.sataraensis, on disturbance is recorded and tested for significance. The surveys have shown sighting of this species only to the perennial streams of the rocky outcrops in Satara region. The potential threats to this species and the possible conservation status based on known distribution are discussed.
Tetragnathid spiderlaterite rocksstreamsseasonal surveyspopulation marginalisationendangered speciesBombay Natural History Society (BNHS), Mumbai- Salim Ali Nature Conservation Fund (SANCF)Introduction
The genus Tylorida Simon, 1894 was established on the basis of a male Tyloridastriata (Thorell, 1877) whose holotype depository details are unknown (Álvarez-Padilla and Hormiga 2011). There are ten currently described species for Tylorida (World Spider Catalog 2014). Most of these are distributed in Australasia except for Tyloridaseriata Thorell, 1899 that extends to West Africa and Cameroon (Álvarez-Padilla and Hormiga 2011). To date, three species of Tylorida are reported from India; Tyloridaculta (O. P.-Cambridge, 1869), Tyloridasataraensis Kulkarni, 2014 and Tyloridaventralis (Thorell, 1877). Tyloridaculta is also reported from Sri Lanka and T.ventralis has a wider distribution from India, to Taiwan, Japan and New Guinea.
Materials and methods
Seven streamside transect surveys of up to 400m were conducted for spiders during 2012, 2013 and 2014. Specimens were collected by visual searching from perennial streams among secondary montane forest along T.sataraensis type locality; Chalkewadi (17.478°N, 73.836°E; 1078m ASL) and Kaas (17.721°N, 73.808°E; 1123m ASL) plateaus of Satara district, India. Males of the species were confirmed by observing copulation. Specimens were collected on private land whilst engaged on permitted surveys for other fauna. Specimens were examined using a Brunel IMXZ™stereozoom microscope and imaged using Canon 1200D™ mounted camera. Statistics were performed in Statsoft Statistica Ver. 7.0™ (StatSoft 2011). Mapping was prepared using DIVA GIS™ v7.5c. All specimens were deposited at Bombay Natural History Society, Mumbai. All the morphological measurements are in millimeters. Abbreviations used: AME - anterior median eyes, PME - posterior median eyes, CDBP - Cymbial dorsobasal process, ASL - Above sea level, BNHS - Bombay Natural History Society, IUCN - International Union for Conservation of Nature.
Total length: 8.1-9.7; carapace: 3.91-4.27 long, 2.31-2.48 wide; abdomen: 3.89-4.01 long, 1.78-1.89 wide. Body pattern in male similar to its female (Kulkarni 2014) (Figs 6, 7). Cephalothorax. Cephalic and thoracic region in same plane. Lateral eyes encircled black, placed on prominent tubercles. AME separated by its diameter, PME less than its diameter. Smooth black pubescence on carapace. Thoracic region margined dark black. Chelicerae brown, long with three promarginal and four retromarginal teeth. Labium brown, semi-circular with slight corrugations; endites longer than wide, with wider proximal edge. Sternum brown, overall heart-shaped with straight margin at coxa II and folded inwards at coxae III and IV. Legs yellow coloured, femora black distally. Abdomen oval shaped, narrower than cephalothorax and slightly overlapping thoracic region when viewed laterally. Dorsum covered with greenish pubescence; venter black with thick yellow lines on the margin, sparsely covered with silver specks and whitish pubescence.
Cymbial dorso-basal process is shorter than half the cymbial width and perpendicular to cymbium longitudinal axis. Embolar base roughly circular. Long macrosetae on palpal metatarsus measuring half times its length. Morphometry of palpal organs is given in Table 1.
Remarks
The variation in body size and patterns in T.ventralis (see Jäger and Praxaysombath 2009) may be confused with T.sataraensis. Male palp and female epigyne examination can confirm the species identity. Furthermore, we found webs of T.sataraensis exclusively above and across stream water surfaces whereas webs of T.ventralis were found either adjacent to stream margins or away, among low vegetation; both observed in different localities.
Diagnosis
Tyloridasataraensis is closely related to T.ventralis but distinguished from all described Tylorida species by the following combination of characters: less swollen tegulum ventrally, longer embolar tip (Figs 1, 2, 3), presence of macroseta on palpal patella (Fig. 4). CDBP erect and pointed in T.sataraensis but slightly bent upwards near tip in T.ventralis (Fig. 5). Paracymbium arrow shaped, lateral margins curved, distal end folded inwards in former but with straight lateral margins and distal edge bulged at one end in latter species. Overall, T.sataraensis is large sized species than T.ventralis (Table 1).
Distribution
India, Maharashtra, Satara.
Discussion
A total of 309 T.sataraensis were observed from three sample surveys across eleven rocky outcrop sites in the northern Western Ghats (Table 2, Fig. 8). Tyloridasataraensis was observed only on high altitude plateaus (≈1100 m ASL) inhabiting surface waters of streams shaded by marginal vegetation. All spiders (N=309) were encountered in webs constructed across the channel width of streams. Orb webs were built with silk lines attached to marginal vegetation and laterite rocks. Webs were built high enough (up to 60 cm) and wide enough (up to 100 cm) to avoid fluctuations in stream water levels. Observationally, laterite rocks seemed to be the preferred anchor points for web construction (N=309) and such microhabitat features may influence web site selection for the species. Some specimens (N=56) were also observed hiding on the side of laterite rocks that faced the stream edge during the monsoon. Spiders that were observed on the sides of laterite rocks seemed to prefer using cavities in the rock surface (N=37) for refuge (Fig. 9). Egg sacs (N=12) were laid pre-monsoon, above stream flow, on the side of laterite rocks (Fig. 10). Spiders escaped into the main flow of stream water when disturbed but were anchored by a dragline to adjacent laterite rocks that assisted their return to the web. Air pockets were observed along the body surface of spiders submerged in stream water (Fig. 11) and it is possible such adaptation might assist them to resurface. Twenty spiders were timed underwater displayed significantly different submersion times (t = 5.78, df = 19, P<0.05). An average of 300 seconds was recorded and the most tenacious remained submerged for over 829 seconds (>13 minutes) (Suppl. material 1).
Conservation status
The greatest current threats to rocky plateau areas continue to be habitat degradation, destruction and direct removal of laterite rocks for construction purposes (Watve 2013). Among those threats observed in the study area, loss of vegetation cover and removal of laterite rocks are likely to be direct threats to T.sataraensis. Such microhabitat degradation, if permitted to continue, could reduce the amount of shaded areas and alter the micro-strata required by the species for web construction. Gradual loss of marginal vegetation along streams could also displace spiders and reduce available microhabitat across sites. Where these threats occur, spiders do congregate in patchy pockets where shade is optimal. Whether this is a natural clustering of population or a response to threats/ impacts remains unknown. If such marginalisation of populations are caused by anthropocentric impacts it could potentially fragment populations in the longer term. Local use of stream water for agricultural purposes is also reducing flow levels across some sites. This action could also affect distribution of spiders across each site and alter niche availability.
In total, ten plateaus were surveyed across the breadth of the northern Western Ghats. From these sites, T.sataraensis has been observed only at Chalkewadi (type-locality) and Kaas plateaus. Combined these plateaus span an area of only 69 Km2. Therefore, in the interests of conservation, we collected only five adult males during the three years survey. Dispersal of these spiders on Chalkewadi and Kaas was observed only during the monsoon period when streams swell and become connected. The extent of occurrence (EOO <100 Km2) and area of occupancy (AOO <10 Km2) for T.sataraensis appears to be fragmented. Based on its current known distribution, the species would likely fall under IUCN status Critically Endangered (CR) following Criteria B1ab(iii)+ B2ab(iii) (IUCN 2012). The detection of this species so far, at just two localities in the northern Western Ghats, and the observed threats recorded here, suggest that T.sataraensis deserves urgent efforts to conserve it.
Acknowledgements
Thanks to Fernando Alvarez-Padilla, Hemant Ghate, Gustavo Hormiga, David Court, Akio Tanikawa, Neelesh Dahanukar for suggestions and helpful discussions, Atul Vartak for sharing photographs, Vishal Deshpande for collecting the first specimen from Chalkewadi, Vinayak Patil for a T.ventralis male specimen, Sameer Padhye, Priyanka Kulkarni for help in preparing plates and Swara Yadav for assitance during the last field survey.
Author contributions
Siddharth Kulkarni was involved in the field work, examining specimens and manuscript writing, Todd Lewis was involved in the data analysis and manuscript writing.
ReferencesÁlvarez-PadillaFernandoHormigaGustavo2011Morphological and phylogenetic atlas of the orb-weaving spider family Tetragnathidae (Araneae: Araneoidea)Zoological Journal of the Linnean Society1624713879http://dx.doi.org/10.1111/j.1096-3642.2011.00692.x10.1111/j.1096-3642.2011.00692.xIUCN2012IUCN Red List Categories and CriteriaSecond editionIUCNGland, Switzerland and Cambridge, UK32JägerP.PraxaysombathB.2009Spiders of Laos: New species and new records (Arachnida: Araneae)Acta Arachnologica582751KulkarniSiddharth2014A new species of the genus Tylorida Simon, 1894 (Araneae: Tetragnathidae) from a rocky outcrop in the northern Western Ghats, IndiaJournal of Threatened Taxa6355585561http://dx.doi.org/10.11609/JoTT.o3606.5558-6110.11609/jott.o3606.5558-61StatSoft2011Statistica (Data Analysis Software System)http://www.statsoft.comVersion 10WatveAparna2013Status review of Rocky plateaus in the northern Western Ghats and Konkan region of Maharashtra, India with recommendations for conservation and managementJournal of Threatened Taxa5539353962http://dx.doi.org/10.11609/JoTT.o3372.3935-6210.11609/jott.o3372.3935-62CatalogWorld Spider2014World Spider Catalog. Natural History Museum Bern2014-12-01T00:00:00+02:00http://wsc.nmbe.chVersion 15.5
Right palp, dorsal view of (a) T.ventralis and (b) T.sataraensis (Scale = 1mm).
Right palp, ventral view of (a) T.ventralis and (b) T.sataraensis (Scale = 1mm).
Conductor and embolar tip in (a) T.ventralis and (b) T.sataraensis (Scale = 0.5 mm).
Macroseta on palpal patella in Tyloridasataraensis.
CDBP pointed in (a) T.ventralis and (b) T.sataraensis (Scale = 1mm).
Habitus dorsal view of Tyloridasataraensis.
Habitus ventral view of Tyloridasataraensis.
Points showing surveyed rocky outcrop sites in Northern Western Ghats, of which sighting of T.sataraensis in red square - Chalkewadi and red circle - Kaas.
Tylorida sataraensis in a cavity of laterite boulder.
Female Tylorida sataraensis on an egg sac.
Air trapped on spider body surface.
Morphometry for diagnosis of male T.sataraensis (N=6) compared to T.ventralis (N=6).
Character
Tylorida ventralis
Tylorida sataraensis
Macroseta on palpal patella
No
Yes
Palpal femora: length to width ratio
9
10-10.5
Tibia length to width ratio
1.15
1.5
Paracymbium to CDBP ratio (range)
0.60-0.75
0.95-1
Embolus: CDBP ratio
0.5
0.97-1
Total size (range in mm)
6.17-7.58
8.1-9.7 (larger species)
Leg I length to carapace length ratio (range)
10.2- 11.71
6.9-7.6
Abundance from three sample surveys of T.sataraensis.
Survey
Males
Females
Juveniles
∑
Jan-14
8
31
64
103
Jun-14
10
18
56
84
Sep-14
39
64
19
122
∑
57
113
139
309
Submersion time of T. sataraensis under water (N=20)