Introduction
The Holarctic genus Peyerimhoffia Kieffer, 1903 was defined by Vilkamaa and Hippa (2005), who presented a key and described several new species. Since then, additional closely related species have been placed within Peyerimhoffia (Hippa and Vilkamaa 2005, Vilkamaa et al. 2013, Shi et al. 2014, Rudzinski and Baumjohann 2009, Rudzinski and Baumjohann 2012) including species formerly included in the Corynoptera crassistylata group sensu Menzel and Mohrig (2000). However the concept of the genus in its current state is disputed with authors continuing to include crassistylata group species in Corynoptera (Menzel et al. 2011, Shin et al. 2013). Until a new analysis of the phylogenetic relationships between representatives is presented, we follow the concept of Vikamaa and Hippa. The following paper presents a new, slightly deviant addition to the genus, Peyerimhoffia jaschhoforum sp. n. and with coeval description of a Northern European subspecies, Peyerimhoffia jaschhoforum fennoscandica ssp. n.
Materials and methods
Thirty five specimens of the new species were collected in Ontario, Canada. Nine male specimens were collected from a jack pine (Picea banksiana) log (Fig. 1a) on the 22 July 2013 and again on the 6 August 2013 located in a closed canopy jack pine forest (47.572 -82.859) near Chapleau, Ontario, Canada. The log (29 cm ø) was in the early stages of decay (decay class 1 based on Rouvinen et al. 2002), with well intact bark. Six other specimens were collected from a similar log (decay class 1, 22cm ø) in the same forest. Two specimens were collected on the 22 July 2013, 6 August 2013 and 19 August 2013 respectively. All specimens were collected using in-situ photoeclectors (Fig. 1b) identical to those described in (Work and Hibbert 2011). Twenty male specimens were also collected from 70 cm log sections taken from a neighboring closed canopy jack pine forest (47.636 -83.243). These specimens were reared from logs in sonotubes between 19 May and 14 September 2013. Eighteen specimens were reared from a log section in advanced stages of decay (decay class 4, 9.5 cm ø). Two additional specimens were reared from separate log sections in advanced stages of decay (decay class 4, 17 cm ø). These specimens were collected from a broader study examining the ecological impacts of intensive biomass harvesting on saproxylic biodiversity. European specimens were collected by Catrin and Mathias Jaschhof in boreal mixed forests during expeditions in Northern Europe. Two specimens were taken with an aspirator and one with a sweep net.
Specimens were sorted using Nikon SMZ800 or Hertel & Reuss STE-5R stereo microscopes and stored in 70% ethanol. Type specimens were selected, dehydrated in 96% ethanol, dissected and slide mounted in Euparal or in Canada Balsam. Specimens were observed under an ISO9001 compound microscope with magnifications of 40×, 100× and 400×.
Specimens were photographed using a MCA-510 USB microscope camera by TUCSEN (Xintu Photonics Co., Ltd.). Between 15–40 images taken at different focal lengths were merged with the aid of the Public Domain Software CombineZP using the method “Weighted Average”. Using GIMP software version 2.8.0., the colour images were converted to greyscale, contrast, and brightness were enhanced and a filter was applied to accentuate the outlines. After manual redrawing of the printed images and a subsequent greyscale scanning at 600 DPI, the final retouch was accomplished again using GIMP. We used scanning electron microscopy (Hitachi S-3400N Scanning Electron Microscope) to characterize the hypopygium. Prior to taking photos, the male gonostyli were dissected from the gonocoxites in 70% alcohol, transferred to 96% ethanol and dried. Gonostyli and gonocoxites were mounted on a single 12.7 mm aluminium specimen stub with epoxy resin and coated with platinum in preparation for secondary electron imaging. All photos were taken at an 11–19 mm working distance from the specimen. Species descriptions were prepared using DELTA (DEscription Language for TAxonomy) (Dallwitz et al. 1999). The following acronyms correspond to the museums and collections where specimens reside: CNC – Canadian National Collection of Insects, Ottawa, Canada; MZHF – Finnish Museum of Natural History (Zoological Museum), University of Helsinki, Helsinki, Finland; SDEI – Senckenberg Deutsches Entomologisches Intitut, Müncheberg, Germany; PWMP – Private Collection of Werner Mohrig, Poseritz, Germany; PKHH – Private collection of Kai Heller, Quickborn, Germany; PRDM – Private collection of Rob Deady, UQÁM, Montréal.